Greame Quest is a hematopathologist at Kingston Health Science Centre and medical director of the flow cytometry laboratory. His areas of academic focus are in flow cytometry and molecular hematopathology, particularly their integration in the diagnosis, prognosis, and predictive assessment of hematologic dyscrasias, as well as assessment of measurable residual disease.
Flow cytometry is a powerful tool for assessing hematolymphoid populations – but with great power comes great responsibility! This sessions will begin with a review the principles of flow cytometry with attention to assessing the quality of data and gating, build in assessment of normal hematolymphoid populations, and then review some classic aberrant immunophenotypes and their disease associations.
1. Identify the technical components of flow cytometry.
2. Assess the technical quality of flow cytometry data.
3. Interpret and correlate multiparameter cytometry data.
4. Integrate clinical and other laboratory data with flow cytometry to formulate a diagnosis