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Quality Assurance in Immunohistochemistry


February 01 2023


10:10 am - 10:55 am


  • Dr. Kelly Dakin Hache
    Dr. Kelly Dakin Hache

    Kelly Dakin Hache is an anatomical pathologist at the QE II Health Sciences Centre in Halifax (Nova Scotia Health Authority – Central Zone). She specializes in orthopedic pathology and breast pathology. She is the medical director of the immunohistochemistry laboratory. She completed a BSc at Mount Allison University, a PhD at Queens University, Medicine and Residency at Dalhousie University and a fellowship in Orthopedic Pathology at the University of Chicago Medical Centre.

This presentation will discuss the immunohistochemical test in terms of the pre-analytical, analytical, and post-analytical variables that influence the test. The importance of appropriate selection of on-slide controls for test calibration and monitoring of test performance will be explained. Test calibration and validation will be reviewed. The importance of external quality control will be discussed.  

At the end of the sessions, participants will be able to: 

  • List variables that influence the results of immunohistochemical tests.
  • Understand selection of appropriate normal tissue controls.
  • Identify specific recommendations for use of positive and negative control tissues.
  • Understand principles of test calibration and validation.
  • Recognize importance of external quality control.

Target Audience: Faculty, Residents

CanMEDS Roles: Medical Expert (the integrating role), Scholar